Brine Shrimp Lab Katie Morton
Recognizing the Danger of
Household Chemicals Using
Brine Shrimp
Katie Morton per. 2
11/19/14
Purpose:
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Recognize the possible dangers of household pollutants.
Use the scientific method to design an experiment to test chemical pollutants.
Measure the effect of various toxic materials on brine shrimp.
Measure using scientific units.
Operate scientific equipment.
Practice basic laboratory safety.
Conduct a scientific investigation as a team.
Learn about the basic biology of brine shrimp.
Hypothesis
In this lab I hypothesize that the most lethal household chemical is bleach and that is has the lowest LD50.
Materials:
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1000 ml beaker/2 gallon tank air pump air stone tubing eye droppers 10 ml pipettes graduated cylinders small petri dishes dissecting scopes/hand lenses beakers stopwatch live brine shrimp
Lysol
Bleach vinegar acetone (nail polish remover)
Procedures:
Place live brine shrimp in a small aquarium tank or a 1000ml beaker with an aerator.
Place 2 petri dishes at each lab table. Three tables will be working on sample A and the other half working on sample B.
Label each dish . For example at lab table #1 there would be one dish labeled 5% and one labeled 10% for sample A and at lab table #2 there would be one dish labeled 20%
and 30% for sample A and so on for the 40% and 50%. At the other lab tables you would label the dishes for sample B.
The setup would appear as below. Lab table #1 Lab table #2 Lab table #3
Sample A
Dish 1 = 5%
Dish 2 = 10% Sample A
Dish 3 = 20%
Dish 4 = 30% Sample A
Dish 5 = 40%
Dish 6 = 50% Lab table #4 Lab table #5 Lab table #6
Sample B
Dish 1 = 5%
Dish 2 = 10% Sample B
Dish 3 = 20%
Dish 4 = 30% Sample B
Dish 5 = 40%
Dish 6 = 50% Add the following to each plate:
5% = 9.5 ml of brine shrimp water + 10 living brine shrimp
10% = 9 ml of brine shrimp water + 10 living brine shrimp
20% = 8 ml of
Household Chemicals Using
Brine Shrimp
Katie Morton per. 2
11/19/14
Purpose:
●
●
●
●
●
●
●
●
Recognize the possible dangers of household pollutants.
Use the scientific method to design an experiment to test chemical pollutants.
Measure the effect of various toxic materials on brine shrimp.
Measure using scientific units.
Operate scientific equipment.
Practice basic laboratory safety.
Conduct a scientific investigation as a team.
Learn about the basic biology of brine shrimp.
Hypothesis
In this lab I hypothesize that the most lethal household chemical is bleach and that is has the lowest LD50.
Materials:
●
●
●
●
●
●
●
●
●
●
●
1000 ml beaker/2 gallon tank air pump air stone tubing eye droppers 10 ml pipettes graduated cylinders small petri dishes dissecting scopes/hand lenses beakers stopwatch live brine shrimp
Lysol
Bleach vinegar acetone (nail polish remover)
Procedures:
Place live brine shrimp in a small aquarium tank or a 1000ml beaker with an aerator.
Place 2 petri dishes at each lab table. Three tables will be working on sample A and the other half working on sample B.
Label each dish . For example at lab table #1 there would be one dish labeled 5% and one labeled 10% for sample A and at lab table #2 there would be one dish labeled 20%
and 30% for sample A and so on for the 40% and 50%. At the other lab tables you would label the dishes for sample B.
The setup would appear as below. Lab table #1 Lab table #2 Lab table #3
Sample A
Dish 1 = 5%
Dish 2 = 10% Sample A
Dish 3 = 20%
Dish 4 = 30% Sample A
Dish 5 = 40%
Dish 6 = 50% Lab table #4 Lab table #5 Lab table #6
Sample B
Dish 1 = 5%
Dish 2 = 10% Sample B
Dish 3 = 20%
Dish 4 = 30% Sample B
Dish 5 = 40%
Dish 6 = 50% Add the following to each plate:
5% = 9.5 ml of brine shrimp water + 10 living brine shrimp
10% = 9 ml of brine shrimp water + 10 living brine shrimp
20% = 8 ml of