Lab Enzymes
Introduction
How does changing the surroundings of enzymes affect their reaction rate? The purpose of the experiment is to determine how different abiotic conditions affect the rate at which enzymes accelerate/cause reactions In this lab students measured the height of the foam after catalysis between catalase (enzyme) and 7 other (solutions) to determine which solution had the fastest reaction rate.. The control variable of the experiment would be the solution of only hydrogen peroxide, water, and catalase. The independent variables of the experiment were the abiotic factors such as PH level, temperature, and the amount of salt within the environment. The dependent variable of the experiment would be the height of the foam(product) after each change in environment. If I change the environment of the catalyst by adding high sodium, low sodium, and very low sodium into three individual test tubes , and measured the height of the foam then low sodium would have the highest reaction rate, this is because changes in the concentration of salt alter the electrostatic interactions between charged amino acids, so if salt is added the ability of enzymes to bind to the substrate is altered and the enzyme may or may not be able to bind to it. If I change the environment of the catalyst by adding room temperature , boiling , and freezing cold Solutions into three individual test tubes and measured the height of the foam then freezing cold Solution would have the highest rate of reaction this is because the higher the temperature the weaker the hydrogen bond. If I change the environment of the catalyst by adding acidic and basic solution into two individual test tubes and measured the height of the foam then basic would have the highest rate of reaction because the PH level also alters the electrostatic interactions doing the same as salt, when PH level is decreased the negative charge is neutralized, hydrogen bind to the unoccupied pair of electrons on the nitrogen
How does changing the surroundings of enzymes affect their reaction rate? The purpose of the experiment is to determine how different abiotic conditions affect the rate at which enzymes accelerate/cause reactions In this lab students measured the height of the foam after catalysis between catalase (enzyme) and 7 other (solutions) to determine which solution had the fastest reaction rate.. The control variable of the experiment would be the solution of only hydrogen peroxide, water, and catalase. The independent variables of the experiment were the abiotic factors such as PH level, temperature, and the amount of salt within the environment. The dependent variable of the experiment would be the height of the foam(product) after each change in environment. If I change the environment of the catalyst by adding high sodium, low sodium, and very low sodium into three individual test tubes , and measured the height of the foam then low sodium would have the highest reaction rate, this is because changes in the concentration of salt alter the electrostatic interactions between charged amino acids, so if salt is added the ability of enzymes to bind to the substrate is altered and the enzyme may or may not be able to bind to it. If I change the environment of the catalyst by adding room temperature , boiling , and freezing cold Solutions into three individual test tubes and measured the height of the foam then freezing cold Solution would have the highest rate of reaction this is because the higher the temperature the weaker the hydrogen bond. If I change the environment of the catalyst by adding acidic and basic solution into two individual test tubes and measured the height of the foam then basic would have the highest rate of reaction because the PH level also alters the electrostatic interactions doing the same as salt, when PH level is decreased the negative charge is neutralized, hydrogen bind to the unoccupied pair of electrons on the nitrogen