Received 3 October 2002/Accepted 21 November 2002 Downloaded from http://aem.asm.org/ on June 6‚ 2013 by UNIVERSITY OF DELHI Despite their commercial importance‚ there are relatively few facile methods for genomic manipulation of the lactic acid bacteria. Here‚ the lactococcal group II intron‚ Ll.ltrB‚ was targeted to
Premium DNA Gene expression RNA
The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
Premium Bacteria Microbiology Staining
Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
Premium DNA Bacteria Gene
sterile swab obtained sample of bacteria from skin then transferred using the aseptic transfer technique into the sterile media. Incubated both specimens for 48 hours observed and recording results of growth at 24 and 48 hours. After observing final growth pattern at 48 hours prepared both wet mount and direct stain slide for each of the cultures. Viewed under microscope using both the 40X and 100X oil immersion lens. Disinfected work area. Observations: |Bacteria
Premium Bacteria
Ferland‚ Rivier University MICRO 206‚ March 16th‚ 2016 Introduction: An unknown bacterium experiment‚ this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al.‚ 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by means of the Embden-Meyerhof pathway‚ they produce plasmid-encoded proteins
Premium Bacteria Microbiology Escherichia coli
Many types of projects are possible when you are able to count bacteria. For example‚ you could count the bacteria in drinking water‚ fresh milk‚ old milk that is slightly sour‚ buttermilk‚ yogurt‚ mud puddle‚ lemonade‚ and many other things. Or you may want to know how fast Chlorox kills bacteria. If you add some Chlorox to a culture and count the surviving bacteria at suitable intervals you can plot killing the killing curve and determine whether the killing is single hit or higher order. It will
Premium Milk Water Technical drawing
Flesh-eating bacteria strikes in Florida On June 16th‚ 26 year-old Cason Yeager went for a swim in Florida in Hernando County and later died from contracting the flesh-eating bacteria‚ Vibrio Vulnificus. This bacteria can be found worldwide‚ most particularly in the warm coastal regions but there have been reports in other parts of the world in non-warm waters. According to the CDC in 2013‚ the case count of the vibrio vulnificus was 104 in thirteen states with six hospitalizations. No deaths‚ but
Premium Bacteria Immune system Escherichia coli
Nowadays‚ Computers have become the main aspects of our life There are so many reasons to why we are so dependent on the computer. Isn’t it unbelievable that some of our parents had never seen a computer before? In the present generation‚ there are computers at almost every corner in the world. There are computers at malls and offices‚ and almost every house in the world. Almost every single person who you know is in possession of a laptop. A computer can perform almost any task that requires even
Premium Mobile phone Personal computer Time
Bacteria‚ such as Vibrio natriegens‚ are single cellular‚ microscopic microorganisms. Bacteria grow by cell division‚ mainly by a process called binary fission‚ where two cells arise from one single cell (Madigan et al.‚ 2015). In bacteria such as Vibrio natriegens‚ who are curved-rod shaped microorganisms‚ they elongate to almost twice their own size and form a dividing wall in which splits the single cell into two daughter cells (Madigan et al.‚ 2015). There are four phases to bacterial cell growth:
Premium Bacteria Bacterial growth
discovered by German Bacteriologist Theodore von Escherich in 1885(quote). It is commonly known as E. coli and 0157:H7; E. coli found in the human intestinal tract and is found in multiple forms. E. coli is rod shaped and the size of two microns to one micron. Widely known for the deadly stain found in feces and meat. The pathogen is still a major instrumental issue in human health and is easily infectious with contaminated sustenance. E. coli is gram-negative bacilli tested in clinical settings from stool
Premium Bacteria Nutrition Escherichia coli